Chicken CCL4 (MIP-1 beta) ELISpot

Catalog Number:
SPOT2390C
Availability:
In stock
Application:
ELISpot
100% Homology:
Gallus gallus (chicken)

Components

UsageQuantityComponent
Capture Antibody100 µgPB0390C-100
Detection Antibody50 µg X 2PBB0398C-050
Standard Control5 µgRP0064C-005
  • The Chicken CCL4 ELISpot assay is designed to evaluate CCL4-secreting cells by sensitively detecting and quantifying low-frequency chemokine-producing cells at the single-cell level. CCL4, also known as macrophage inflammatory protein-1 beta (MIP-1β), is a chemokine that plays an important role in recruiting and activating immune cells such as monocytes, macrophages, T lymphocytes, and natural killer cells to sites of infection and inflammation. In chickens, CCL4 contributes to regulation of innate and inflammatory immune responses and supports leukocyte migration during host defense against pathogens. Measurement of CCL4 is particularly important in avian immunology research for studying immune responses to infectious diseases such as avian influenza virus, Newcastle disease virus, Marek's disease virus, and other respiratory or systemic infections affecting poultry. This assay enables researchers to quantify CCL4-producing cells to better understand chemokine-mediated immune cell recruitment and inflammatory responses in chickens. The Chicken CCL4 ELISpot kit contains a Capture Antibody, Detection Antibody, and Standard Control sufficient for the development of up to two (2) ELISpot assays. The included antibodies have been validated for performance in an ELISpot assay using the provided Standard Control. Additional materials and reagents required to complete the assay are not included. Individual components may also be purchased separately. Made in the USA. For research use only.
Storage Conditions2°C to 8°C
Country Of OriginUSA
Chicken CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta–like chemokine in birds) is a pro-inflammatory chemokine involved in recruitment and activation of immune cells during inflammatory and infectious responses in chickens (Gallus gallus). CCL4 functions through chemokine receptors homologous to mammalian CCR5, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer–like cells, and activated T lymphocytes to sites of infection or tissue injury. In chickens, CCL4 is produced by activated macrophages, epithelial cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecular patterns and cytokines including IL-1β and TNF-like mediators. CCL4-mediated immune cell recruitment plays an important role in host defense against pathogens responsible for avian influenza, Newcastle disease, infectious bronchitis, and bacterial infections such as Salmonella and Mycoplasma gallisepticum. By directing leukocyte trafficking to infected tissues, CCL4 helps coordinate innate and adaptive immune responses and contributes to pathogen clearance. However, excessive or prolonged expression may contribute to inflammatory tissue damage. As a regulator of leukocyte migration and inflammatory signaling, chicken CCL4 is important for studies of avian host–pathogen interactions, immune regulation, and vaccine responses, supporting research aimed at improving disease resistance and flock health in poultry production systems.
Price: $1,200.00

Reagents for Animal Model and Animal Health Research

Kingfisher Biotech, Inc.
1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

Animal Antibodies

Newsletter

"