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Bovine CD45R0 Monoclonal Antibody (clone ILA116A)

WS0593B-100
$200.00
In Stock
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Background

CD45 is a type 1 transmembrane molecule found on the surface of all nucleated hematopoietic cells and their precursors, except for mature erythrocytes and platelets. It is one of the most abundant cell surface glycoproteins.The cytoplasmic region is 95% homologous across mammalian species. CD45 is expressed in various isoforms. Expression of different CD45 isoforms is cell type specific, and depends on the state of activation and differentiation of hematopoietic cells.

Specifications

Clone - ILA116A

Isotype - IgG3

Host - Mouse

Known Reactivity - Bovine, Caprine, Ovine

Purification - Anti-bovine CD45R0 monoclonal antibody, clone ILA116A, was produced in ascites fluid, clarified by filtration through a 0.2 micrometer filter.

Alternate Names

Flow cytometric profile obtained with bovine leukocytes.
WS0593B

Catalog No.:
WS0593B-100
Quantity:
100 ug
Country of Origin:
USA
Host:
Mouse
Applications:
The Bovine CD45R0 monoclonal antibody, clone ILA116A, has been qualified for use in flow cytometry applications.

31695097

Expansion, isolation and first characterization of bovine Th17 lymphocytes.

Cunha P, Vern YL, Gitton C, Germon P, Foucras G, Rainard P.

Sci Rep. 2019 Nov 6;9(1):16115. doi: 10.1038/s41598-019-52562-2.

Applications: Isolation of CD4+ cells; Measurement of CD45RO by flow cytometric analysis; Staining of IL-17A

Abstract

Interleukin 17A-producing T helper cells (Th17) are CD4+ T cells that are crucial to immunity to extracellular bacteria. The roles of these cells in the bovine species are poorly defined, because the characterization of bovine Th17 cells lags behind for want of straightforward cultivation and isolation procedures. We have developed procedures to differentiate, expand, and isolate bovine Th17 cells from circulating CD4+ T cells of adult cows. Using polyclonal stimulation with antibodies to CD3 and CD28, we expanded IL-17A-positive CD4+ T cells in a serum-free cell culture medium supplemented with TGF-β1, IL-6 and IL-2. Populations of CD4+ T cells producing IL-17A or IFN-γ or both cytokines were obtained. Isolation of IL-17A-secreting CD4+ T cells was performed by labelling surface IL-17A, followed by flow cytometry cell sorting. The sorted Th17 cells were restimulated and could be expanded for several weeks. These cells were further characterized by cytokine profiling at transcriptomic and protein levels. They produced high amounts of IL-17A and IL-17F, and moderate amounts of IL-22 and IFN-γ. The techniques developed will be useful to characterize the phenotypic and functional properties of bovine Th17 cells.

 


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