Multi-species (Ovine, Caprine) IL-8 ELISA

Catalog Number:: DIY1030V
Availability:: In stock
Application:: ELISA
100% Homology:: Capra hircus (goat), Ovis aries (sheep), Ovis canadensis (bighorn sheep)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	PBB0266S-050
Standard	5 µg	RP0488V-005
Capture Antibody	100 µg	PB0143S-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	PB0143S-100	1 Pack (<= 20 Plates)

Product Description

The Ovine and Caprine IL-8 (Interleukin-8, also known as CXCL8) ELISA is designed for the quantitative measurement of IL-8 in cell culture supernatants from sheep and goats, and can also be used to measure IL-8 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, bronchoalveolar lavage fluid, synovial fluid, and tissue lysates from organs such as lung, spleen, liver, and lymph nodes, supporting a broad range of immunology, inflammation, and translational research applications. IL-8 is a chemokine primarily produced by macrophages, epithelial cells, and endothelial cells, and it plays a central role in recruiting neutrophils, promoting inflammatory responses, and coordinating innate immune defense. In sheep and goat models, IL-8 is widely studied in the context of bacterial and viral infections, respiratory diseases, mastitis, and inflammatory disorders, where its expression reflects immune activation and neutrophil recruitment. Measurement of IL-8 provides valuable insight into immune regulation, disease progression, and the efficacy of immunomodulatory interventions. Reported measurements of ovine and caprine IL-8 have detected basal serum and plasma levels of approximately 20-250 pg/mL, with elevated levels-often 300-2,000 pg/mL-following infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from inflamed or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-8 in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Background Info

Ovine and Caprine Interleukin-8 (IL-8), also known as CXCL8, is a highly conserved pro-inflammatory chemokine that plays a central role in innate immune responses in sheep (Ovis aries) and goats (Capra hircus), with the IL-8 protein sequence being identical between the two species. Produced by macrophages, epithelial cells, endothelial cells, and mammary gland cells in response to bacterial, viral, and inflammatory stimuli, IL-8 signals through CXCR1 and CXCR2 receptors to mediate neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Elevated IL-8 expression is associated with economically significant small ruminant diseases including mastitis, pneumonia, caseous lymphadenitis, enteric infections, foot rot, and parasitic infestations, where neutrophil-driven inflammation contributes to both pathogen clearance and tissue pathology. In the mammary gland, IL-8 is a key mediator of somatic cell recruitment during intramammary infection and serves as an early biomarker of mastitis severity and therapeutic response. In animal health and translational research, characterization of ovine/caprine IL-8 supports studies of mucosal immunity, respiratory disease, mammary gland inflammation, and host-pathogen interactions in small ruminants, while also providing comparative insight into neutrophil-mediated inflammatory mechanisms relevant to human respiratory and inflammatory diseases.

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