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Swine Fas Ligand (TNFSF6) ELISA
- Catalog Number:
- DIY1149S
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Sus scrofa (pig)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1148S-050 | |
| Standard | 5 µg | RP1068S-005 | |
| Capture Antibody | 100 µg | KP1147S-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1147S-100 | 1 Pack (<= 20 Plates) |
- The Swine Fas Ligand (FasL) ELISA is designed for the quantitative measurement of FasL in cell culture supernatants from pigs and can also be used to measure FasL in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, lymph node and spleen tissue lysates, and tissue extracts from organs such as lung, liver, and intestine, supporting a broad range of immunology, apoptosis, and translational research applications. FasL is a member of the TNF superfamily primarily expressed by activated T cells, natural killer (NK) cells, and certain immune-activated tissue cells, and it plays a central role in inducing apoptosis in Fas-expressing target cells, regulating immune homeostasis, and controlling cytotoxic responses. In the swine animal model, FasL is widely studied in the context of immune-mediated cell death, transplant immunology, viral infection, and autoimmune disorders, where its expression reflects the regulation of apoptosis and immune cell-mediated cytotoxicity. Measurement of FasL provides valuable insight into cell death signaling, immune regulation, disease progression, and the efficacy of immunomodulatory or therapeutic interventions. Reported measurements of swine FasL have detected basal serum and plasma levels of approximately 50-500 pg/mL, with elevated levels-often 600-3,000 pg/mL-following immune stimulation, infection, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring FasL in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Swine Fas Ligand (FasL, CD95L, gene FASLG) is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, TRAIL (TNFSF10), and CD40L (CD154) that regulate immune signaling, inflammation, and apoptosis. In pigs (Sus scrofa), Fas ligand is primarily expressed on activated T lymphocytes, cytotoxic T cells, and natural killer (NK) cells, where it interacts with its receptor Fas (CD95) on target cells. Binding of FasL to Fas activates the extrinsic apoptotic pathway, triggering caspase-mediated signaling cascades that induce programmed cell death. In swine immunity, the Fas-FasL system plays an important role in cytotoxic T-cell-mediated elimination of virus-infected or abnormal cells and in maintaining immune homeostasis by removing activated or autoreactive lymphocytes. Because pigs share important physiological and immunological similarities with humans, they are widely used as translational animal models for immunology and transplantation research, and Fas-FasL signaling has been studied in porcine models of organ transplantation, where Fas-mediated apoptosis contributes to regulation of alloreactive immune responses and mechanisms of graft rejection or tolerance, providing insights relevant to human transplant immunology and immune regulation.
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