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Swine IL-1 alpha ELISA
- Catalog Number:
- DIY1172S
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Phacochoerus africanus (Common warthog), Sus scrofa (pig)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1171S-050 | |
| Standard | 5 µg | RP1035S-005 | |
| Capture Antibody | 100 µg | KP1170S-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1170S-100 | 1 Pack (<= 20 Plates) |
- The Swine IL-1α (Interleukin-1 alpha) ELISA is designed for the quantitative measurement of IL-1α in cell culture supernatants from swine and can also be used to measure IL-1α in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, bronchoalveolar lavage fluid, and tissue lysates from organs such as spleen, lung, liver, and lymph nodes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-1α in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-1α is a pro-inflammatory cytokine produced by monocytes, macrophages, epithelial cells, and fibroblasts, and it plays a critical role in the initiation of inflammatory responses, activation of innate immunity, and regulation of leukocyte recruitment. In the swine animal model, IL-1α is widely studied in the context of infectious disease, respiratory inflammation, vaccine response, tissue injury, and immune modulation, where it serves as an early marker of inflammatory activation. Measurement of IL-1α provides valuable insight into local and systemic inflammation, cytokine signaling pathways, disease progression, and the efficacy of anti-inflammatory or immunomodulatory interventions, with reported levels typically detected at low picogram-per-milliliter concentrations under basal conditions and increasing during acute inflammatory or infectious challenge, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Swine Interleukin-1 Alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family-which includes IL-1α, IL-1β, and the IL-1 receptor antagonist (IL-1Ra)-and plays a central role in initiating innate and sterile inflammatory responses in pigs (Sus scrofa domesticus). IL-1α is constitutively expressed in epithelial and barrier tissues and functions as an intracellular alarmin that is rapidly released upon cell injury, necrosis, or viral infection, triggering early inflammatory signaling independent of inflammasome processing. Upon binding to the IL-1 receptor type 1 (IL-1R1), swine IL-1α activates NF-κB and MAPK pathways that promote leukocyte recruitment, endothelial activation, fever, and amplification of pro-inflammatory cytokine cascades. In swine health, IL-1α is particularly relevant in respiratory and enteric diseases such as porcine reproductive and respiratory syndrome (PRRS), swine influenza, African swine fever, bacterial pneumonia, and intestinal infections, where epithelial damage leads to early IL-1α release and contributes to lung or gut inflammation. While essential for pathogen defense and tissue repair, excessive IL-1α signaling can exacerbate tissue injury and impair growth performance. As an early mediator of sterile and infectious inflammation, swine IL-1α serves as a biomarker of tissue damage and innate immune activation. In translational research, pigs are valuable large-animal models due to physiological similarities to humans, and characterization of swine IL-1α supports studies of cytokine-driven inflammation, sepsis, trauma, and development of targeted IL-1-modulating therapies relevant to both veterinary and human medicine.
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