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Bovine CD40 Ligand (TNFSF5) ELISA
- Catalog Number:
- DIY1211B
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Bison bison bison (American buffalo), Bos mutus (wild yak), Bos taurus (cattle)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1210B-050 | |
| Standard | 5 µg | RP1008B-005 | |
| Capture Antibody | 100 µg | KP1209B-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1209B-100 | 1 Pack (<= 20 Plates) |
- The Bovine CD40 Ligand (CD40L, also known as CD154) ELISA is designed for the quantitative measurement of CD40L in cell culture supernatants from cattle and can also be used to measure CD40L in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as spleen, lymph nodes, and thymus. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring CD40L in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. CD40L is a transmembrane protein primarily expressed by activated bovine T lymphocytes and plays a central role in B cell activation, immunoglobulin class switching, dendritic cell maturation, and the regulation of adaptive immune responses. In the bovine animal model, CD40L is widely studied in the context of immune responses to infection, vaccination, and inflammatory or autoimmune conditions, where its expression reflects T cell activation and immune system modulation. Measurement of bovine CD40L provides valuable insight into immune activation, cytokine signaling, adaptive immune regulation, and the efficacy of immunotherapeutic or vaccine interventions, with reported levels in cattle typically detected at low picogram-per-milliliter concentrations under basal conditions (~20-150 pg/mL in serum or plasma) and increasing to several hundred to over a thousand picograms per milliliter in response to immune stimulation or inflammatory challenge, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant bovine CD40L protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Bovine CD40 Ligand (CD40L), also known as CD154 and encoded by the CD40LG gene, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, CD27 ligand (CD70), OX40 ligand (CD252), and 4-1BB ligand (CD137L) that regulate immune activation and cell-cell signaling. In cattle (Bos taurus), CD40L is primarily expressed on activated CD4⁺ T lymphocytes, and can also be detected on platelets and other activated immune cells. CD40L binds to its receptor CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells, initiating intracellular signaling pathways such as NF-κB and MAPK that promote B-cell activation, immunoglobulin class switching, germinal center formation, and the development of memory B cells. Through these mechanisms, bovine CD40L plays a central role in T cell-dependent antibody responses and regulation of adaptive immunity. In cattle, the CD40-CD40L signaling pathway contributes to immune responses against viral, bacterial, and parasitic pathogens, including those involved in bovine respiratory disease, mastitis, and other infectious conditions. Because CD40L is critical for T-cell-mediated B-cell help and effective vaccine-induced immunity, bovine CD40L is studied in veterinary immunology, vaccine development, and host-pathogen interaction research, helping to improve strategies for disease control and immune protection in cattle.
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