Chicken IFN gamma Recombinant Protein

Catalog Number:: RP0115C
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Gallus gallus (chicken)

Chicken IFN-γ (catalog RP0115C) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.7 kDa, 145 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to chicken (note: this material contains an Ile73 to Thr substitution - a corrected matching the published sequence is available as catalog RP0929C). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study interferon-γ signaling, macrophage activation, Th1 immune responses, antigen presentation and antiviral/immunoregulatory functions; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	HTASSLNLVQ LQDDIDKLKA DFNSSHSDVA DGGPIIVEKL KNWTERNEKR IILSQIVSMY LEMLENTDKS KPHTKHISEE LYTLKNNLPD GVKKVKDIMD LAKLPMNDLR IQRKAANELF SILQKLVDPP SFKRKRSQSQ RRCNC (145)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	16.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Chicken Interferon-Gamma (IFN-γ) is the sole type II interferon in chickens (Gallus gallus) and is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes as well as natural killer (NK)-like cells, where it plays a central role in cell-mediated (Th1-type) immunity. IFN-γ signals through the IFN-γ receptor complex (IFNGR1/IFNGR2), activating JAK/STAT1 pathways and inducing genes involved in macrophage activation, enhanced antigen presentation via upregulation of MHC class I and II molecules, and promotion of cytotoxic T-cell responses. In poultry health, IFN-γ is critical for defense against intracellular pathogens and economically significant diseases such as avian influenza virus, Newcastle disease virus, Marek's disease virus, Salmonella spp., Eimeria spp. (coccidiosis), and Mycobacterium avium, where robust Th1 responses contribute to pathogen control and vaccine-induced protection. Altered or insufficient IFN-γ responses can impact disease susceptibility, growth performance, and flock health. In research settings, chickens serve as important models for avian viral oncogenesis (Marek's disease), mucosal immunity, and vaccine development, and characterization of chicken IFN-γ supports studies of T-cell biology, host-pathogen interactions, immune correlates of protection, and comparative cell-mediated immunity relevant to both veterinary and broader immunological research.

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