Mouse APRIL Recombinant Protein

Catalog Number:
RP0360M
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus caroli (Ryukyu mouse), Mus musculus (house mouse)
  • Mouse APRIL (catalog RP0360M) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.4 kDa, 146 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with amino-acid homology to mouse (Mus musculus) and Mus caroli. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study APRIL/TACI/BCMA signaling, B-cell survival, plasma-cell differentiation and antibody biology; typical experimental uses include cell-culture stimulation and dosing studies, B-cell proliferation and differentiation assays, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAVLTQKHKKK HSVLHLVPVN ITSKADSDVT EVMWQPVLRR GRGLEAQGDI VRVWDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIRSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL (146)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight16.4 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the tumor necrosis factor (TNF) superfamily and plays a key role in B cell biology and humoral immunity. APRIL is produced primarily by myeloid cells such as monocytes, macrophages, and dendritic cells, as well as by some epithelial and stromal cells, and it signals through the receptors TACI (TNFRSF13B) and BCMA (TNFRSF17) to promote B cell survival, plasma cell maintenance, and immunoglobulin class switching. In mice, APRIL contributes to the regulation of antibody production, long-lived plasma cell persistence in the bone marrow, and mucosal IgA responses. Dysregulated APRIL expression has been associated with autoimmune disease models, B cell malignancies, and chronic inflammatory conditions. In biomedical research, mouse APRIL is widely studied in models of systemic lupus erythematosus (SLE), rheumatoid arthritis, multiple myeloma, and antibody-mediated immune responses, where it serves as an important mediator of B cell–driven immunity and a potential therapeutic target for modulating pathological antibody production.

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