Rabbit IFN gamma ELISA

Catalog Number:: DIY0713U
Application:: ELISA
100% Homology:: Oryctolagus cuniculus (rabbit)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1309U-050
Standard	5 µg	RP0136U-005
Capture Antibody	100 µg	KP1308U-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1308U-100	1 Pack (<= 20 Plates)

Product Description

The Rabbit IFN-γ (Interferon Gamma) ELISA is designed for the quantitative measurement of rabbit IFN-γ in cell culture supernatants. With minor modifications to the assay procedure, this ELISA can also be used to measure IFN-γ in additional biological sample types reported in the literature, including serum, plasma, tissue lysates, and bronchoalveolar lavage fluid, supporting a wide range of immunology and translational research applications. IFN-γ is a central Th1 cytokine produced primarily by activated T cells and NK cells and is a key regulator of cell-mediated immunity. It plays a critical role in macrophage activation, antigen presentation, and antiviral and antimicrobial defense. Measuring rabbit IFN-γ provides essential insight into Th1-driven immune responses, making it a valuable biomarker for assessing immune activation, protective immunity, and immune dysregulation. In rabbit models, IFN-γ is widely used to evaluate infectious disease progression, vaccine efficacy, inflammatory responses, and immunotherapeutic interventions, where changes in IFN-γ levels reflect both the strength and quality of the immune response. Reported measurements of rabbit IFN-γ have detected low to moderate picogram per milliliter concentrations in serum and plasma under baseline conditions, with significantly elevated levels observed following immune stimulation, infection, or vaccination. IFN-γ has also been measured in tissue lysates and localized samples from inflamed or infected sites, reflecting site-specific immune activation. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties/Specifications

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Rabbit Interferon-γ (IFN-γ) is a type II interferon cytokine that plays a central role in cell-mediated immunity and regulation of antimicrobial and inflammatory responses in rabbits (Oryctolagus cuniculus). IFN-γ is produced primarily by activated T helper 1 (Th1) cells, cytotoxic T lymphocytes, and natural killer (NK) cells following stimulation by intracellular pathogens or immune signaling molecules such as IL-12 and IL-18. Rabbit IFN-γ binds to the interferon-γ receptor (IFNGR1/IFNGR2) expressed on many immune and non-immune cells, activating intracellular signaling through the JAK–STAT pathway, particularly STAT1, which induces expression of numerous interferon-stimulated genes involved in antigen presentation, macrophage activation, and antimicrobial defense. In rabbits, IFN-γ is critical for activation of macrophages, enhancement of MHC class I and II expression, and control of intracellular pathogens including viruses, bacteria, and parasites. Rabbit IFN-γ is widely studied in rabbit models of infectious disease and immunology; for example, IFN-γ responses are commonly measured in rabbit models of Mycobacterium tuberculosis infection, where IFN-γ–producing T cells serve as an important indicator of cellular immune responses and disease progression.