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Guinea Pig IL-8 ELISA
- Catalog Number:
- DIY0933GP
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Cavia porcellus (domestic guinea pig)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | PBB0314U-050 | |
| Standard | 5 µg | RP0344GP-005 | |
| Capture Antibody | 100 µg | PB0313U-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | PB0313U-100 | 1 Pack (<= 20 Plates) |
- The Guinea Pig IL-8 (Interleukin-8, also known as CXCL8) ELISA is designed for the quantitative measurement of IL-8 in cell culture supernatants from guinea pigs and can also be used to measure IL-8 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, bronchoalveolar lavage fluid, synovial fluid, and tissue lysates from organs such as lung, spleen, liver, and lymph nodes, supporting a broad range of immunology, inflammation, and translational research applications. IL-8 is a chemokine primarily produced by macrophages, epithelial cells, and endothelial cells, and it plays a central role in recruiting and activating neutrophils, promoting inflammatory responses, and coordinating innate immune defense. In the guinea pig model, IL-8 is widely studied in the context of bacterial and viral infections, inflammatory disorders, and pulmonary disease, where its expression reflects immune activation and inflammatory signaling. Measurement of IL-8 provides valuable insight into immune cell recruitment, disease progression, and the efficacy of immunomodulatory interventions. Reported measurements of guinea pig IL-8 have detected basal serum and plasma levels of approximately 20-250 pg/mL, with elevated levels-often 300-2,000 pg/mL-following infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from inflamed or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Guinea Pig Interleukin-8 (IL-8), also known as CXCL8, is a pro-inflammatory chemokine that plays a central role in innate immune responses in guinea pigs (Cavia porcellus) by mediating neutrophil chemotaxis, activation, and recruitment to sites of infection or tissue injury. Produced by macrophages, epithelial cells, and endothelial cells in response to microbial products and inflammatory cytokines, guinea pig IL-8 signals through CXCR1 and CXCR2 receptors to drive neutrophil migration and amplification of local inflammatory responses. IL-8 is significantly upregulated in pulmonary tissues during infections such as Mycobacterium tuberculosis, influenza virus, and other respiratory pathogens, contributing to granulocytic infiltration and lung pathology. Because guinea pigs are well-established models for tuberculosis, influenza, cytomegalovirus, and certain hemorrhagic fever viruses, characterization of IL-8 is critical for understanding neutrophil-driven inflammation, granuloma formation, and cytokine-mediated tissue damage. As both a biomarker and functional mediator of acute inflammation, guinea pig IL-8 supports translational research in respiratory disease, vaccine development, and host-pathogen interaction studies relevant to human infectious and inflammatory disorders.
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