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Caprine IL-6 ELISA
- Catalog Number:
- DIY1316G
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Capra hircus (goat)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB0653B-050 | |
| Standard | 5 µg | RP1071G-005 | |
| Capture Antibody | 100 µg | KP0652B-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP0652B-100 | 1 Pack (<= 20 Plates) |
- The Caprine IL-6 (Interleukin-6) ELISA is designed for the quantitative measurement of IL-6 in cell culture supernatants from goats (Capra hircus) and can also be used to measure IL-6 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as spleen, lymph nodes, liver, and mammary gland. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-6 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-6 is a pleiotropic cytokine produced primarily by activated macrophages, T cells, and endothelial cells and plays a central role in inflammation, immune regulation, hematopoiesis, and acute phase responses. In the caprine animal model, IL-6 is widely studied in the context of infectious disease, mastitis, vaccination, and immunomodulatory research, where its expression reflects inflammatory and immune activation. Measurement of IL-6 provides valuable insight into immune responses, cytokine signaling, disease progression, and the efficacy of therapeutic or vaccine interventions, with reported levels in goats typically detected at low nanogram-per-milliliter concentrations under basal conditions (~0.2-2 ng/mL in serum or plasma) and increasing during immune challenge, infection, or inflammatory stimulation, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant caprine IL-6 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Caprine Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine and a central member of the IL-6 cytokine family—including IL-6, IL-11, IL-27, IL-31, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and cardiotrophin-like cytokine factor 1 (CLCF1)—which signal through receptor complexes utilizing the shared gp130 subunit to activate JAK/STAT and MAPK pathways. In goats (Capra hircus), IL-6 plays a key role in regulating acute-phase protein production (such as haptoglobin and serum amyloid A), leukocyte activation, B- and T-cell differentiation, and systemic inflammatory responses during infection, tissue injury, and metabolic stress. Elevated IL-6 expression is associated with economically significant caprine diseases including mastitis, caprine arthritis encephalitis (CAE), caseous lymphadenitis, pneumonia, enteric infections, and parasitic infestations, where it contributes to fever, local inflammation, and disease severity. As a biomarker, caprine IL-6 is valuable for monitoring herd health, assessing vaccine responses, and evaluating therapeutic interventions. In animal health and translational research, characterization of caprine IL-6 and related family members supports studies of host-pathogen interactions, inflammatory regulation in small ruminants, comparative immunology, and the development of immune-based strategies to improve productivity, welfare, and disease resistance in goat populations.
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