Mouse Oncostatin M (OSM) ELISA

Catalog Number:: DIY1438M
Application:: ELISA
100% Homology:: Mus musculus (house mouse)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1437M-050
Standard	5 µg	RP1222M-005
Capture Antibody	100 µg	KP1436M-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1436M-100	1 Pack (<= 20 Plates)

Product Description

The Mouse Oncostatin M (OSM) ELISA is designed for the quantitative measurement of Oncostatin M in cell culture supernatants from mice (Mus musculus) and can also be used to measure OSM in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as lung, liver, spleen, and joint tissue. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring OSM in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. Oncostatin M is a pleiotropic cytokine produced primarily by activated macrophages, neutrophils, T cells, and stromal cells and is a member of the IL-6 cytokine family, playing a key role in inflammation, tissue remodeling, fibrosis, hematopoiesis, and bone metabolism. In the mouse animal model, Oncostatin M is widely studied in the context of chronic inflammatory disease, arthritis, pulmonary fibrosis, cardiovascular research, and cancer, where its expression reflects inflammatory signaling and tissue remodeling processes. Measurement of Oncostatin M provides valuable insight into cytokine signaling pathways, disease mechanisms, and the evaluation of anti-inflammatory or anti-fibrotic therapeutic strategies, with reported levels in mice typically detected at low picogram-per-milliliter concentrations under basal conditions (~10-150 pg/mL in serum or plasma) and increasing during immune activation, tissue injury, or disease progression, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant mouse Oncostatin M protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties/Specifications

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Mouse Oncostatin M (OSM) is a multifunctional cytokine belonging to the IL-6 cytokine family, which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), ciliary neurotrophic factor (CNTF), cardiotrophin-like cytokine factor 1 (CLCF1), and IL-27. In mice (Mus musculus), OSM signals through receptor complexes containing the shared gp130 (IL6ST) subunit together with the oncostatin M receptor (OSMR), activating downstream signaling pathways including JAK/STAT (particularly STAT3), MAPK, and PI3K/AKT. Murine OSM is produced primarily by activated T lymphocytes, macrophages, neutrophils, and dendritic cells and plays roles in regulating inflammation, hematopoiesis, immune responses, and tissue remodeling. In mouse models, OSM has been widely studied in experimental systems of inflammatory disease, fibrosis, and cancer, where it influences cytokine production, fibroblast activation, and extracellular matrix remodeling. OSM signaling is particularly relevant in murine models of rheumatoid arthritis, inflammatory bowel disease, lung inflammation, and liver fibrosis, where it contributes to chronic inflammatory signaling and tissue pathology. Because mice are the most widely used genetic model organism in biomedical research, characterization of mouse OSM has been instrumental in understanding IL-6 family cytokine signaling, STAT3-mediated inflammatory pathways, and mechanisms of tissue remodeling, providing insights relevant to human inflammatory and fibrotic diseases.