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CartCaprine LIF Do-It-Yourself ELISA, ≤10 Plates
Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.
The Caprine LIF-A Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Caprine LIF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Caprine LIF-A Do-It-Yourself ELISA can also be used to measure Chiru LIF.
| Component | Usage | Quantity | Catalog # |
| Anti-Human LIF Polyclonal Antibody | Capture Antibody | 100 µg | KP1486H-100 |
| Biotinylated Anti-Human LIF Polyclonal Antibody | Detection Antibody | 50 µg | KPB1487H-050 |
| Caprine LIF Recombinant Protein | Standard | 5 µg | RP1075G-005 |
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Kingfisher Biotech, Inc.
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USA
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Kingfisher Biotech brand products are warranted by Kingfisher Biotech, Inc. to meet stated product specifications and to conform to label descriptions when used, handled and stored according to instructions. Unless otherwise stated, this warranty is limited to one year from date of sale. Kingfisher Biotech’s sole liability for the product is limited to replacement of the product or refund of the purchase price. Kingfisher Biotech brand products are supplied for research applications. They are not intended for medicinal, diagnostic or therapeutic use. The products may not be resold, modified for resale or used to manufacture commercial products without prior written approval from Kingfisher Biotech.
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