Bovine Erythropoietin (EPO) ELISA

Catalog Number:: DIY1585B
Application:: ELISA
100% Homology:: Bison bison bison (American buffalo), Bos grunniens (domestic yak), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1584B-050
Standard	5 µg	RP1275B-005
Capture Antibody	100 µg	KP1583B-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1583B-100	1 Pack (<= 20 Plates)

Product Description

The Bovine EPO (Erythropoietin) ELISA is designed for the quantitative measurement of EPO in cell culture supernatants from cattle (Bos taurus) and can also be used to measure EPO in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, kidney tissue lysates, and bone marrow samples. While the assay is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring EPO in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. EPO is a glycoprotein hormone produced primarily by renal peritubular interstitial cells in response to hypoxia, and it plays a central role in erythropoiesis by promoting the proliferation, differentiation, and survival of erythroid progenitor cells in the bone marrow through signaling via the erythropoietin receptor (EPOR). In the bovine animal model, EPO is studied in the context of anemia, hypoxic stress, high-altitude adaptation, neonatal development, production health, and renal function, making it an important biomarker in both veterinary medicine and livestock management. Measurement of bovine EPO provides valuable insight into oxygen-sensing pathways, red blood cell production, and physiological adaptation to stress, with reported concentrations typically detected at low picogram-per-milliliter levels under normal physiological conditions and increasing in response to hypoxia, anemia, hemorrhage, or renal stimulation, depending on sample type and clinical context. The kit contains a capture antibody, detection antibody, and recombinant bovine EPO protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties/Specifications

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Bovine erythropoietin (EPO) is a glycoprotein hormone produced primarily by the kidneys in response to hypoxia that regulates erythropoiesis by binding to the erythropoietin receptor (EPOR) on erythroid progenitor cells, activating JAK2/STAT5, PI3K/Akt, and MAPK signaling pathways to promote red blood cell survival, proliferation, and differentiation. In cattle, EPO is essential for maintaining adequate oxygen-carrying capacity during growth, pregnancy, lactation, high-altitude adaptation, and recovery from blood loss or disease. In bovine health, EPO plays a critical role in the response to anemia associated with parasitic infections, chronic inflammation, nutritional deficiencies, hemorrhage, and renal disease, making it an important biomarker and potential therapeutic target for improving animal welfare and productivity. Beyond livestock health, bovine EPO is valuable in comparative physiology and translational research, serving as a large-animal model for investigating erythropoiesis, hypoxia signaling, renal endocrine function, and the development of erythropoiesis-stimulating therapies for human anemia and related hematologic disorders.