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Feline Erythropoietin (EPO) ELISA
- Catalog Number:
- DIY1587F
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Felis catus (domestic cat)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1584B-050 | |
| Standard | 5 µg | RP1524F-005 | |
| Capture Antibody | 100 µg | KP1583B-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1583B-100 | 1 Pack (<= 20 Plates) |
- The Feline EPO (Erythropoietin) ELISA is designed for the quantitative measurement of EPO in cell culture supernatants from cats (Felis catus) and can also be used to measure EPO in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, kidney tissue lysates, and bone marrow samples. While the assay is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring EPO in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. EPO is a glycoprotein hormone produced primarily by renal peritubular interstitial cells in response to hypoxia, where it regulates erythropoiesis by promoting proliferation, differentiation, and survival of erythroid progenitor cells in the bone marrow through activation of the erythropoietin receptor (EPOR). In the feline animal model, EPO is widely studied in the context of chronic kidney disease-associated anemia, hypoxic stress, hemorrhage, neonatal development, and hematologic disorders, making it a critical biomarker in veterinary internal medicine and translational renal research. Measurement of feline EPO provides valuable insight into oxygen-sensing pathways, red blood cell production, and disease progression, with reported concentrations typically detected at low picogram-per-milliliter levels under normal physiological conditions and increasing in response to anemia, hypoxia, blood loss, or renal dysfunction, depending on sample type and clinical context. The kit contains a capture antibody, detection antibody, and recombinant feline EPO protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Feline Erythropoietin (EPO) is a glycoprotein hormone belonging to the type I cytokine family that regulates erythropoiesis, the production of red blood cells. In cats (Felis catus), erythropoietin is produced primarily by peritubular interstitial cells in the kidney, with smaller amounts produced in the liver, in response to hypoxia or reduced oxygen-carrying capacity of the blood. Feline EPO binds to the erythropoietin receptor (EPOR) on erythroid progenitor cells in the bone marrow, activating signaling pathways such as JAK2-STAT5, PI3K-AKT, and MAPK, which promote survival, proliferation, and differentiation of erythroid precursor cells into mature red blood cells. In feline health, erythropoietin is essential for maintaining normal hematocrit and oxygen delivery to tissues, and insufficient EPO production is a major contributor to anemia associated with chronic kidney disease (CKD), a common condition in aging cats. Cats also serve as clinical models for studying anemia of chronic kidney disease, and research on feline erythropoietin has contributed to understanding the pathophysiology of renal anemia and the therapeutic use of recombinant erythropoietin or EPO-stimulating agents to manage anemia in both veterinary and human medicine.
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