Canine CXCL10 (IP-10) ELISA

Catalog Number:: DIY1665D
Availability:: In stock
Application:: ELISA
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1664D-050
Standard	5 µg	RP0754D-005
Capture Antibody	100 µg	KP1663D-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1663D-100	1 Pack (<= 20 Plates)

Product Description

The Canine CXCL10 (IP-10) ELISA is designed for the quantitative measurement of CXCL10 in cell culture supernatants from dogs (Canis lupus familiaris) and can also be applied to other sample types reported in the literature, including serum, plasma, peripheral blood leukocyte culture supernatants, and tissue lysates, with minor protocol adjustments required for optimal performance in different matrices. CXCL10 is a CXC chemokine produced by activated monocytes, macrophages, dendritic cells, endothelial cells, and other immune cells in response to interferon-gamma and inflammatory stimuli, functioning primarily through the CXCR3 receptor to recruit and activate T cells, NK cells, and other leukocytes at sites of infection or tissue inflammation. In canine models, CXCL10 plays a critical role in cell-mediated immunity, antiviral responses, and inflammatory signaling, making it relevant for studying viral infections, autoimmune conditions, and vaccine-induced immune activation. Measurement of canine CXCL10 provides insight into chemokine-driven immune cell trafficking, Th1-type immune responses, and host-pathogen interactions, with concentrations typically low under basal conditions and increasing following infection, vaccination, or cytokine stimulation depending on tissue source and experimental design. The kit contains a capture antibody, detection antibody, and recombinant canine CXCL10 protein standard validated for use together in an ELISA format, with components available separately if desired; this product is for research use only and is manufactured in the USA.

Properties

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Background Info

Canine CXCL10 (C-X-C motif chemokine ligand 10), also known as IP-10 (Interferon gamma-induced protein 10), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL9 (MIG) and CXCL11 (I-TAC) that regulate T-cell recruitment during antiviral and inflammatory immune responses. In dogs (Canis lupus familiaris), CXCL10 is produced by macrophages, dendritic cells, epithelial cells, endothelial cells, and fibroblasts in response to interferon-γ (IFN-γ), viral infection, or inflammatory cytokines. CXCL10 signals primarily through the chemokine receptor CXCR3, which is expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and activation of these cells at sites of infection or inflammation. In canine health, CXCL10 contributes to immune responses against viral, bacterial, and parasitic pathogens, and elevated CXCL10 expression has been observed in inflammatory and immune-mediated diseases, including canine leishmaniasis, where recruitment of Th1-type T cells is important for parasite control. Dogs also serve as comparative animal models for human infectious and immune-mediated diseases, and canine leishmaniasis is widely studied as a model for human visceral leishmaniasis, where CXCL10-mediated recruitment of CXCR3⁺ T cells is associated with protective cell-mediated immunity and disease progression, making canine CXCL10 relevant for studies of host-pathogen interactions, immune regulation, and therapeutic development.

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